Moloney Murine Leukemia Virus Reverse Transcriptase

RNase H Minus, Point Mutant

Available formats SKU# Price (SGD) ^

  • 2,500 units D001-01a S$ 45.00

  • 10,000 units D001-01b S$ 160.00

  • 2,500 units D001-01c S$ 580.00

^ All prices are subject to prevailing GST

* Custom sizes can be provided upon request.

Manufactured at Cellbae’s Singapore facilities, which are certified to ISO 13485:2016 Medical Devices ‒ Quality Management Systems


Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus, Point Mutant is an RNA-dependent DNA polymerase that synthesizes, from a primer, a complementary DNA strand using RNA as a template. The RNase H inactivation improves synthesis of full-length cDNA, enhances thermostability, and increases the affinity to the template-primer.


  • Increased thermostability

The possibility of a higher incubation temperature is associated with reduction of early terminations from RNA secondary structure and reduced non-specific priming.

  • Improved full-length cDNA yield

Inactivation of the RNase H domain precludes the degradation of the sample RNA by the RT.


  • First-strand DNA synthesis

  • Primer extension

  • Reverse transcriptase for regular end-point and real-time RT-PCR


Product Source

  • Recombinant expression in E. coli. The enzyme contains 13 additional amino acids at the N-terminus and 10 amino acids at the C-terminus.


  • 240 units/µL

Storage Buffer

  • 50 mM Tris-HCl (pH 8 at RT)

  • 150 mM NaCl

  • 1 mM DTT

  • 0.1 mM EDTA

  • 0.1% IGEPAL

  • 50% glycerol

Enzyme Purity

  • Single band as judged by SDS-polyacrylamide gel electrophoresis.

Free of Host DNA

  • No host DNA detected as determined by RT-PCR in at least 480 units.

Free of RNases

  • Integrity of RNA sample >80% after incubation with 480 units of enzyme for 30 min at 37 °C.

Storage Temperature

  • -25 to -15 °C