H⁻ MMLV RT
Moloney Murine Leukemia Virus Reverse Transcriptase
RNase H Minus, Point Mutant
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Available formats SKU# Price (SGD) ^
2,500 units D001-01a S$ 45.00
10,000 units D001-01b S$ 160.00
40,000 units D001-01c S$ 580.00
^ All prices are subject to prevailing GST
* Custom sizes can be provided upon request.
INTENDED USE
INTENDED USE
Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus, Point Mutant is an RNA-dependent DNA polymerase that synthesizes, from a primer, a complementary DNA strand using RNA as a template. The RNase H inactivation improves synthesis of full-length cDNA, enhances thermostability, and increases the affinity to the template-primer.
KEY HIGHLIGHTS
KEY HIGHLIGHTS
Increased thermostability
The possibility of a higher incubation temperature is associated with reduction of early terminations from RNA secondary structure and reduced non-specific priming.
Improved full-length cDNA yield
Inactivation of the RNase H domain precludes the degradation of the sample RNA by the RT.
APPLICATIONS
APPLICATIONS
First-strand DNA synthesis
Primer extension
Reverse transcriptase for regular end-point and real-time RT-PCR
SPECIFICATIONS
SPECIFICATIONS
Product Source
Recombinant expression in E. coli. The enzyme contains 13 additional amino acids at the N-terminus and 10 amino acids at the C-terminus.
Storage Buffer
50 mM Tris-HCl (pH 8 at RT)
150 mM NaCl
1 mM DTT
0.1 mM EDTA
0.01% IGEPAL
50% glycerol
Enzyme Purity
Single band as judged by SDS-polyacrylamide gel electrophoresis.
Free of Host DNA
No host DNA detected as determined by RT-PCR in at least 480 units.
Free of RNases
Integrity of RNA sample >80% after incubation with 480 units of enzyme for 30 min at 37 °C.
Storage Temperature
-25 to -15 °C
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